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Decoding Stem Cell Biology via Transcriptional Elements: MicroRNAs
Stem cells have been characterized extensively in terms of their gene expression profiles. Recent studies have indicated that microRNA, a class of non-coding small RNA that participate in the regulation of gene expression, may play a key role in stem cell self renewal and differentiation. We seek to identify the differences in microRNA expression between undifferentiated human embryonic stem cells (hESC) and their corresponding differentiated cells that underwent differentiation in vitro.
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Fig. 11. Heat map of microRNA expression in hESC and their corresponding differentiated cells. qPCR of statistically significant microRNA candidates was performed on 3 independent hESC lines each.
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Profiling of the hESC “microRNA-ome” provides an insight into molecules that control cellular differentiation and maintenance of the pluripotent state, findings that have broad implications in development, homeostasis and human disease states. Results from R. Hart (BMB) and collaborators have confirmed the identity of a signature microRNA profile in pluripotent cells, comprising a small subset of differentially expressed microRNAs in hESCs (see Fig. 11). Patterns of expression were identified in the progression from hESC to differentiated cells that suggest a role for selected microRNAs in maintenance of the undifferentiated, pluripotent state. (see color key, inset, Fig. 11). IGERT Trainee projects coadvised by S. Gunderson (BMB), R. Nowakowski (BMB) and I. Androulakis (BME) examine whether developmentally-regulated transcription factors systematically control protein synthesis by traditional regulation of mRNA transcription, by inducing specific, suppressive microRNAs, and by responding to regulated microRNAs. The IGERT project will specifically focus on artificially-controlled differentiation of neural stem cells (NSC), which has potential to produce specific cell types for therapeutic transplant in brain trauma, stroke, spinal cord injury.
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| Major Events |
IRIF:Megan Anderson Fri., 12/11 in BME-122, 12-1 pm
Enhanced Survival of Progeny of Neural Stem Cells in Response to Trace Eyeblink Conditioning
IRIF:Andrew LHuillier Thurs., 11/19 in BME-122, 12-1pm
Mesenchymal Stem Cell Mediated Immunosuppression and IDO Metabolites
RESCHEDULED: Bioindustry Ethics Luncheon Part II w/ David Finegold (IGERT Fellows ONLY) 11/5/09
Part II of the Ethics Luncheon will be rescheduled to the Spring semester IRIF schedule.
IRIF:Dr. Debu Banerjee 10/22 in BME-122, 12-1 pm
Therapeutic applications of bone marrrow derived Mesenchmal stem cells
View all major events >>
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| More News |
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Congratulations!
IGERT Fellows Aaron Carlson and Mohamed Sadik took 1st and 3rd place (respectively) in the Poster Presentations at the 3rd Annual NJ Stem Cell Symposium held Thursday, September 24, 2009. Aaron Carlson's poster was titled “3-D Electrospun Polymer Scaffolds Promote Human Embryonic Stem Cell Self-Renewal and Controlled Organization". Mohamed presented “Electroporation-Mediated Molecular Delivery”. Jonathan Davilla took 2nd place with “Identification of Biologically Functional microRNAs in Human ESCs by Ago2 Immunoprecipitation and Sequencing”. The keynote address was delivered by Hakim Djaballah, PhD of the Memorial Sloan Kettering Cancer Center.
Congratulations to Professor Ki-Bum Lee, Stem Cell IGERT Faculty in Chemistry and Chemical Biology, who is the recipient of the NIH Director’s New Innovator award, 2009.
The NIH Director's New Innovator Award program is designed specifically to support unusually creative early stage investigators with highly innovative research ideas at an early stage of their career.
IGERT fellow wins award:
Congratulations to IGERT fellow Nicole Plourde who was recipient of the 2009 Schering-Plough Innovation Award. She was presented with an awards plaque and a check for $5000 at a ceremony on Thursday, April 16th.
Chris Ricupero featured on Epigenie interview
Follow link here for full interview.
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