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3-D Constructs for Stem Cell Expansion and Differentiation
Despite the promise of obtaining various lineages,hESCs are difficult to maintain and expand over long periods and higher-order (three-dimensional) substrates to support and expand hES cells have not been forthcoming. In this IGERT project area, the Trainees will be advised by J. Kohn (CCB) on the fabrication of 3-D, polymeric microconstructs with extremely high surface area to volume ratios areas (20,000 cm2/g, compared to conventional substrates with 300-4500 cm2/g[30]) and investigate methods for expansion of hESCs in the P. Moghe lab (CBE/BME), and for in situ differentiation-upon demand, in the laboratory of M. Schachner (Neuroscience). The hypothesis is that substrate rigidity, bioadhesivity, and scaffold microstructure can concertedly influence hESC colony outgrowth and influence pluripotency and proliferation behaviors. A parallel goal is to examine up-scaleable approaches to prolong hESC expansion using hES cell-seeded microscaffolds perfused within low-shear bioreactors. Optimized, cell-seeded constructs will be differentiated, and implanted under guidance from M. Grumet (BMB) within cell transplantation models with two explicit goals: identification of hESC constructs that maintain differentiation and limit teratoma formation in vivo. Preliminary results (Fig. 14) indicate that the combination of matrix incorporation with scaffolds may sensitively alter stemness vs. differentiation of human embryonic stem cells.
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Fig. 14. LEFT: hESC adhesion and outgrowth on 3-D microscaffolds was modulated by matrix pretreatment, and, in turn, influenced the qualitative expression of pluripotency (Oct-4) or differentiation (SSEA-1). Laminin and to some degree collagen elicited expression of red SSEA-1, which appears as red and yellow (colocalization), respectively. Fibronectin and untreated scaffolds elicited hESC outgrowth without promoting differentiation. RIGHT: Fraction of hESC colonies expressing SSEA-1 was quantified for a larger set of conditions. |
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| Major Events |
IRIF:Megan Anderson Fri., 12/11 in BME-122, 12-1 pm
Enhanced Survival of Progeny of Neural Stem Cells in Response to Trace Eyeblink Conditioning
IRIF:Andrew LHuillier Thurs., 11/19 in BME-122, 12-1pm
Mesenchymal Stem Cell Mediated Immunosuppression and IDO Metabolites
RESCHEDULED: Bioindustry Ethics Luncheon Part II w/ David Finegold (IGERT Fellows ONLY) 11/5/09
Part II of the Ethics Luncheon will be rescheduled to the Spring semester IRIF schedule.
IRIF:Dr. Debu Banerjee 10/22 in BME-122, 12-1 pm
Therapeutic applications of bone marrrow derived Mesenchmal stem cells
View all major events >>
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| More News |
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Congratulations!
IGERT Fellows Aaron Carlson and Mohamed Sadik took 1st and 3rd place (respectively) in the Poster Presentations at the 3rd Annual NJ Stem Cell Symposium held Thursday, September 24, 2009. Aaron Carlson's poster was titled “3-D Electrospun Polymer Scaffolds Promote Human Embryonic Stem Cell Self-Renewal and Controlled Organization". Mohamed presented “Electroporation-Mediated Molecular Delivery”. Jonathan Davilla took 2nd place with “Identification of Biologically Functional microRNAs in Human ESCs by Ago2 Immunoprecipitation and Sequencing”. The keynote address was delivered by Hakim Djaballah, PhD of the Memorial Sloan Kettering Cancer Center.
Congratulations to Professor Ki-Bum Lee, Stem Cell IGERT Faculty in Chemistry and Chemical Biology, who is the recipient of the NIH Director’s New Innovator award, 2009.
The NIH Director's New Innovator Award program is designed specifically to support unusually creative early stage investigators with highly innovative research ideas at an early stage of their career.
IGERT fellow wins award:
Congratulations to IGERT fellow Nicole Plourde who was recipient of the 2009 Schering-Plough Innovation Award. She was presented with an awards plaque and a check for $5000 at a ceremony on Thursday, April 16th.
Chris Ricupero featured on Epigenie interview
Follow link here for full interview.
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