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Project Theme 12: Ligand Conformational Biosensor Substrates
The development of solid state biosensors, such as sensors for glucose detection and quantification, is key for rapid monitoring of important vital parameters. We are developing a program to design such devices that can quantify a biological property into an electronic signal. For instance, we are designing a device that is based on a large conformational change in Glucose Galactose Binding Protein (GGBP) occurring upon glucose-binding. This conformational change is used to modulate the charge injection into a silicon chip, thereby providing a direct electrical measurement of glucose concentration.
Mutants of GGBP have been prepared that contain cysteine residues. Cysteine provides a reactive thiol group that allows covalent attachment of thiol reactive probes at engineered locations (gln26cys and met182cys). The double cys mutants will be labeled with a donor-acceptor pair of tetramethyl rhodamine and Cy5. The Cy5 is a singlet-excited-state-sensitized electron-acceptor and has a second functional group that allows covalent tethering to the functionalized silicon surface. The rate of promotion of the acceptor to the first singlet state controls the rate of charge-injection into the silicon. The amino acid substitutions are on opposite domains such that the conformational change associated with binding the ligand causes a change in distance between the two probes. This modulates the energy transfer rate to the acceptor. This design does not consume the glucose during its operation. Binding of the glucose is reversible allowing reuse of the sensor. The essential elements of the biosensors that we will study are a diode laser to excite the acceptor, a well-functionalized silicon interface to bind the glucose binding proteins and to harbor a p-i-n junction, and a matrix to localize the active area to insulating part of the junction. This example illustrates the approach necessary to design novel biosensors.
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11:45 am - 1 pm, Room CCR-201 (Note: change of location!) awarded 2-year pre-doctoral fellowship from New Jersey Commission on Cancer Research for his research "Immune Targeting using an Engineered Vaccine Delivery System", advised by Professors Martin Yarmush and Edmund Lattime winners of the Best Poster Awards at the Biointerfacial IGERT Symposium on April 11th Congratulations to: Professor Charlie Roth, selected to receive a Warren L. Susman Award for Excellence in Teaching "for his outdstanding service in stimulating and guiding the intellectual development" of Rutgers students; and to Prof. Noshir Langrana, recipient of a Faculty- Scholar Teacher Award, which honors faculty members whose research "informs and adds excitement to their teaching and ...stimulates and informs students' scholarship." 11:45am-1 pm, Room 122, BME Bldg. Friday April 11, 2008; 8:00 am - 1:30 pm; Busch Dining Hall, Rooms A&B. Research Talks & Posters by IGERT Trainees; Plenary Talk by Prof. Scott Diamond, U. Penn; [Agenda and Roster of Talks and Posters] 11:45 am-1 pm, Room 122, BME Bldg. 11:45 am-1:00 pm, Room 122, BME Bldg. Topic: Novel Anionic Block Copolymers for Oligonucleotide Delivery Presenter: Lavanya Peddada, IGERT Trainee Panelists Tim Maguire (Merck & Co.), Eric Novik (Hurel Corp.), and Bala Subramanian (Goldman Sachs) will discuss early career opportunities for graduate students and postdocs in math, science, and engineering. Monday Nov 26; 5:00 - 6:30 pm; Center Hall, Busch Campus Ctr; sign-up required; call Career Services at (732) 445-6127, ext. 0 Topic: The Effects of Substrate Rigidity on Synaptogenesis and Dendrite Branching Speaker: Michelle Previtera, IGERT Trainee Topic: IGERT on Biointerfaces: Milestones Crossed and New Challenges Ahead Presenter: Professor Prabhas Moghe, IGERT Program Director Topic: Relationship of Chemotherapeutic Dose-Response Curves to Cell Cycle Effects Presenter: Salaheldin Hamed, IGERT Graduate Training Fellow All IGERT Trainees are actively working toward publications. Jose Fernandez and Matthew Treiser, Cohort II/III trainees leading with the most crossdisciplinary publications, will receive the 2007 IGERT Scholarly Excellence Awards. Charles Florek and Nicole Plourde deserve honorary mention. Congratulations to IGERT Graduate Trainee Mike Wininger, selected for the NSF's 2007 East Asian Pacific Summer Institute and awarded an internship for stem cell research at the National Taiwan University. was held on October 20, 2006, featuring presentations by all Trainees and Plenary Lecture by Christopher Chen, MD,PhD; U. Penn. [agenda, talks/posters ] Congratulations to our newest Graduate Training Fellows: Lavanya Peddada, Kevin Nikitczuk, Michelle Previtera and Jocie Harris.
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| Rutgers IGERT on Biointerfaces holds Inaugural Celebration and Research Seminars on January 30, 2004 The Fall 2004 class of IGERT Graduate fellows has been announced. To view information about the new fellows, please click here. Rutgers has been awarded the first NIH postdoctoral program in Tissue Engineering. Rutgers to house the first NIH-resource center for polymeric biomaterials. New Undergraduate Research Frontiers Program, ISURF launched June 2004 Rutgers has been awarded its first and New Jersey\'s second IGERT Grant.(Rutgers Media Relations) (Targum Coverage) |