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Project Theme 13: Gene expression profiling for engineering of cell responses to biointerfaces
Faculty Advisors:
Hart (MBS), Roth (CBE), Dunn (BME)
Crossdisciplinary Bridging:
Molecular Biology, Biomedical Engineering, Statistics, Materials Science
DNA microarrays can have a major impact on the evaluation of biological interfaces due to their ability to provide a comprehensive measurement of gene expression across families of genes promoting favorable cell-biomaterial interactions (e.g., growth, differentiation) and unfavorable responses (e.g., inflammation, apoptosis). The successful application of this technology to biointerfacial engineering necessitates the development of new techniques for data handling and analysis and the integration of gene expression profiles with other biological databases. We will address these needs in the context of important applications in spinal cord regeneration and liver cell-based devices.
Cellular responses to biointerfaces represent a mixture of influences due to the biomaterial and due to soluble factors in its local microenvironment. A pattern recognition technique known as independent components analysis (ICA), frequently used in attribution of sources in EEG data, will be used as a data mining technique to separate these effects in large gene expression datasets (Dunn, Roth). These methods will be tested with data generated using a custom-built, 5,000-gene spotted oligonucleotide microarray (Hart) with fully automated processing capabilities. In one application of this approach, we will study the activation of de-differentiation pathways in hepatocytes and the relationship between gene expression and liver-specific functions in successful vs. less successful culture environments (substrate and medium combinations). Recent results highlight the activation of inflammatory pathways as a source of de-differentiation in hepatocytes.
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| Major Events |
IRIF:Megan Anderson Fri., 12/11 in BME-122, 12-1 pm
Enhanced Survival of Progeny of Neural Stem Cells in Response to Trace Eyeblink Conditioning
IRIF:Andrew LHuillier Thurs., 11/19 in BME-122, 12-1pm
Mesenchymal Stem Cell Mediated Immunosuppression and IDO Metabolites
RESCHEDULED: Bioindustry Ethics Luncheon Part II w/ David Finegold (IGERT Fellows ONLY) 11/5/09
Part II of the Ethics Luncheon will be rescheduled to the Spring semester IRIF schedule.
IRIF:Dr. Debu Banerjee 10/22 in BME-122, 12-1 pm
Therapeutic applications of bone marrrow derived Mesenchmal stem cells
View all major events >>
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| More News |
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Congratulations!
IGERT Fellows Aaron Carlson and Mohamed Sadik took 1st and 3rd place (respectively) in the Poster Presentations at the 3rd Annual NJ Stem Cell Symposium held Thursday, September 24, 2009. Aaron Carlson's poster was titled “3-D Electrospun Polymer Scaffolds Promote Human Embryonic Stem Cell Self-Renewal and Controlled Organization". Mohamed presented “Electroporation-Mediated Molecular Delivery”. Jonathan Davilla took 2nd place with “Identification of Biologically Functional microRNAs in Human ESCs by Ago2 Immunoprecipitation and Sequencing”. The keynote address was delivered by Hakim Djaballah, PhD of the Memorial Sloan Kettering Cancer Center.
Congratulations to Professor Ki-Bum Lee, Stem Cell IGERT Faculty in Chemistry and Chemical Biology, who is the recipient of the NIH Director’s New Innovator award, 2009.
The NIH Director's New Innovator Award program is designed specifically to support unusually creative early stage investigators with highly innovative research ideas at an early stage of their career.
IGERT fellow wins award:
Congratulations to IGERT fellow Nicole Plourde who was recipient of the 2009 Schering-Plough Innovation Award. She was presented with an awards plaque and a check for $5000 at a ceremony on Thursday, April 16th.
Chris Ricupero featured on Epigenie interview
Follow link here for full interview.
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