|
|
Project Theme 3: Biodynamics of Cell Receptor-Mediated Adhesive Interfaces with Matrix Ligands
Faculty Advisors:
Moghe (BME), Riman (CME), Kohn (CCB), Schwarzbauer (Collaborator, Princeton U.)
Crossdisciplinary Bridging:
Cell Biology, Matrix Biology, Polymer Chemistry, Materials Eng., Biomedical Eng.
Cell internalizable ligand interfaces with nanoparticles can serve to achieve unprecedented levels of cellular motility on biomaterials ((21)). The top figure shows a single skin epidermal cell (keratinocyte) with actin-rich filopodial extensions on ligand-derivatized nanoparticles. The lower figure illustrates that cells migrate on such interfaces by clearing the ligand-nanoparticle - thus these internalizable interfaces are biodynamic in nature.Projects in this thrust will focus on altering and tracking ligand sampling dynamics at these interfaces through systematic modifications to biomaterial substrate chemistry, as well as through display of mutated adhesive ligands. The major emphasis in these projects will be on the analysis of the membrane-binding and cytointernalization dynamics of the adhesive ligands displayed from nanocarriers of variable geometries and substrate densities. The key tools utilized include laser-excited multiphoton fluorescence microscopy and image analysis. With a view to discern the ligand-bearing nanocarrier trafficking within individual migrating cells, fluorescent, GFP-actin transfected cells will be used, and ligand nanocarriers will be encapsulated with fluorescent quantum dot nanocrystals. From these studies, minimal ligand fragments can be identified whose display from nanoparticles that can promote ligand sampling dynamics and activate intracellular protein kinases mediating cell migration. Biopolymer substrates will be designed to promote ligand clearance and trafficking dynamics.
|
|
|
|
|
| Major Events |
IRIF:Megan Anderson Fri., 12/11 in BME-122, 12-1 pm
Enhanced Survival of Progeny of Neural Stem Cells in Response to Trace Eyeblink Conditioning
IRIF:Andrew LHuillier Thurs., 11/19 in BME-122, 12-1pm
Mesenchymal Stem Cell Mediated Immunosuppression and IDO Metabolites
RESCHEDULED: Bioindustry Ethics Luncheon Part II w/ David Finegold (IGERT Fellows ONLY) 11/5/09
Part II of the Ethics Luncheon will be rescheduled to the Spring semester IRIF schedule.
IRIF:Dr. Debu Banerjee 10/22 in BME-122, 12-1 pm
Therapeutic applications of bone marrrow derived Mesenchmal stem cells
View all major events >>
|
| More News |
|
|
Congratulations!
IGERT Fellows Aaron Carlson and Mohamed Sadik took 1st and 3rd place (respectively) in the Poster Presentations at the 3rd Annual NJ Stem Cell Symposium held Thursday, September 24, 2009. Aaron Carlson's poster was titled “3-D Electrospun Polymer Scaffolds Promote Human Embryonic Stem Cell Self-Renewal and Controlled Organization". Mohamed presented “Electroporation-Mediated Molecular Delivery”. Jonathan Davilla took 2nd place with “Identification of Biologically Functional microRNAs in Human ESCs by Ago2 Immunoprecipitation and Sequencing”. The keynote address was delivered by Hakim Djaballah, PhD of the Memorial Sloan Kettering Cancer Center.
Congratulations to Professor Ki-Bum Lee, Stem Cell IGERT Faculty in Chemistry and Chemical Biology, who is the recipient of the NIH Director’s New Innovator award, 2009.
The NIH Director's New Innovator Award program is designed specifically to support unusually creative early stage investigators with highly innovative research ideas at an early stage of their career.
IGERT fellow wins award:
Congratulations to IGERT fellow Nicole Plourde who was recipient of the 2009 Schering-Plough Innovation Award. She was presented with an awards plaque and a check for $5000 at a ceremony on Thursday, April 16th.
Chris Ricupero featured on Epigenie interview
Follow link here for full interview.
|
|
