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Project Theme 7: Microfluidics and Microfabricated Substrates for Differentiation Studies of Stem Cells
Faculty Advisors:
Yarmush (BME), Grumet (MBS), Moghe (BME), Uhrich (CCB)
Crossdisciplinary Bridging:
Bioengineering, Cell Biology, Microsystems Engineering, Polymer Chemistry
This project aims to develop robust methodology for deriving (a) functional adult liver cells (hepatocytes) from embryonic stem (ES) cells; and (b) genetically reprogrammed liver cells from differentiated hepatocytes. Studies are underway in the laboratory of Moghe to explore genetic
reprogramming of hepatic-like embryonic stem cells upon exposure to adhesive ligands that activate Wnt-signaling pathways, which modulate the switch between cell growth and differentiation. Strategic function-modulating ligands will be presented from micropatterned polymeric substrates in both basal and lateral configurations, so as to explore the role of "polarity" of ligand microdisplay on cell gene reprogramming. The images shown here illustrate that when hepatocytes are grown on cadherin-derivatized microsphere studded microislands, there is expression of intracellular catenin, a molecular switch for tipping the differentiation-proliferation balance within these cells.
Separately, studies in the Yarmush group are establishing a clear relationship between the differentiation of hepatocytes and the embryonic stem cell chemotactic response to specific environmental stimuli. Using techniques involving microfluidic design, design of bioactive macrofabricated surfaces, and cell micropatterning, microfluidic bioreactors will be studied to determine the conditions that produce optimal ES cell differentiation into functional hepatocytes.
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| Major Events |
IRIF:Megan Anderson Fri., 12/11 in BME-122, 12-1 pm
Enhanced Survival of Progeny of Neural Stem Cells in Response to Trace Eyeblink Conditioning
IRIF:Andrew LHuillier Thurs., 11/19 in BME-122, 12-1pm
Mesenchymal Stem Cell Mediated Immunosuppression and IDO Metabolites
RESCHEDULED: Bioindustry Ethics Luncheon Part II w/ David Finegold (IGERT Fellows ONLY) 11/5/09
Part II of the Ethics Luncheon will be rescheduled to the Spring semester IRIF schedule.
IRIF:Dr. Debu Banerjee 10/22 in BME-122, 12-1 pm
Therapeutic applications of bone marrrow derived Mesenchmal stem cells
View all major events >>
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| More News |
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Congratulations!
IGERT Fellows Aaron Carlson and Mohamed Sadik took 1st and 3rd place (respectively) in the Poster Presentations at the 3rd Annual NJ Stem Cell Symposium held Thursday, September 24, 2009. Aaron Carlson's poster was titled “3-D Electrospun Polymer Scaffolds Promote Human Embryonic Stem Cell Self-Renewal and Controlled Organization". Mohamed presented “Electroporation-Mediated Molecular Delivery”. Jonathan Davilla took 2nd place with “Identification of Biologically Functional microRNAs in Human ESCs by Ago2 Immunoprecipitation and Sequencing”. The keynote address was delivered by Hakim Djaballah, PhD of the Memorial Sloan Kettering Cancer Center.
Congratulations to Professor Ki-Bum Lee, Stem Cell IGERT Faculty in Chemistry and Chemical Biology, who is the recipient of the NIH Director’s New Innovator award, 2009.
The NIH Director's New Innovator Award program is designed specifically to support unusually creative early stage investigators with highly innovative research ideas at an early stage of their career.
IGERT fellow wins award:
Congratulations to IGERT fellow Nicole Plourde who was recipient of the 2009 Schering-Plough Innovation Award. She was presented with an awards plaque and a check for $5000 at a ceremony on Thursday, April 16th.
Chris Ricupero featured on Epigenie interview
Follow link here for full interview.
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